TRAF2 Polyclonal Antibody

$Lane 1: Human Hela cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human THP-1 cell lysates; Lane 4: Rat PC-12 cell lysates; Lane 5: Human Romas cell lysates; Lane 6: Human Jurkat cell lysates; Lane 7: Mouse NIH\/3T3 cell lysates probed with TRAF2 Polyclonal Antibody, Unconjugated (bs-55217R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Hela cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human THP-1 cell lysates; Lane 4: Rat PC-12 cell lysates; Lane 5: Human Romas cell lysates; Lane 6: Human Jurkat cell lysates; Lane 7: Mouse NIH\/3T3 cell lysates probed with TRAF2 Polyclonal Antibody, Unconjugated (bs-55217R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: 293t cell lysates; Lane 2: TRAF2 knockout (KO) 293t cell lysates probed with TRAF2 Polyclonal Antibody, Unconjugated (bs-55217R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TRAF2 ) polyclonal Antibody, Unconjugated (bs-55217R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc$

Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TRAF2 ) polyclonal Antibody, Unconjugated (bs-55217R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc

Applications

WB
IF(ICC)
IP
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55217R
Product Name	TRAF2 Polyclonal Antibody
Applications	WB, IF(ICC), IP, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF2 (NP_066961.2).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7186
Swiss Prot	Q12933
Subcellular location	Cytoplasm
Synonyms	MGC:45012;TRAP;TRAP3;TRAF2
Background	The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IP	1-2ug
IF