ROCK1 Polyclonal Antibody

Lane 1: Human 293T cell lysates; Lane 2: Human U-251MG cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human BT-474 cell lysates probed with ROCK1 Polyclonal Antibody, Unconjugated (bs-55188R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: ROCK1 knockout (KO) HeLa cell lysates probed with ROCK1 Polyclonal Antibody, Unconjugated (bs-55188R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ROCK1 Polyclonal Antibody, Unconjugated (bs-55188R) at 1:100 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ROCK1 Polyclonal Antibody, Unconjugated (bs-55188R) at 1:100 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IP
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55188R
Product Name	ROCK1 Polyclonal Antibody
Applications	WB, IHC-P, IP, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6093
Swiss Prot	Q13464
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ROCK1;P160ROCK;ROCK-I
Background	This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IP	1-2ug
IHC