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HINT1 Polyclonal Antibody

Lane 1: Hela cell lysates; Lane 2: HINT1 knockout (KO) HeLa cell lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: HINT1 knockout (KO) HeLa cell lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 22RV1 cell lysates; Lane 2: Hela cell lysates; Lane 3: H460 cell lysates; Lane 4: K562 cell lysates; Lane 5: Mouse Kidney lysates; Lane 6: Mouse Liver lysates; Lane 7: Rat Kidney lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 22RV1 cell lysates; Lane 2: Hela cell lysates; Lane 3: H460 cell lysates; Lane 4: K562 cell lysates; Lane 5: Mouse Kidney lysates; Lane 6: Mouse Liver lysates; Lane 7: Rat Kidney lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-55097R
Product Name HINT1 Polyclonal Antibody
Applications WB
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HINT1 (NP_005331.1).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 3094
Swiss Prot P49773
Subcellular location Cytoplasm, Nucleus
Synonyms HINT1;HINT;NMAN;PKCI-1;PRKCNH1
Background This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed.
Application Dilution
WB 1:300-5000

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