GSK3B Polyclonal Antibody

Lane 1: LO2 cell lysates; Lane 2: Hela cell lysates; Lane 3: 22Rv1 cell lysates; Lane 4: U251-MG cell lysates probed with GSK3B Polyclonal Antibody, Unconjugated (bs-55090R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: GSK3B knockout (KO) HeLa cell lysates probed with GSK3B Polyclonal Antibody, Unconjugated (bs-55090R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GSK3B ) polyclonal Antibody, Unconjugated (bs-55090R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GSK3B ) polyclonal Antibody, Unconjugated (bs-55090R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IF(ICC)
IP
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-55090R
Product Name	GSK3B Polyclonal Antibody
Applications	WB, IF(ICC), IP, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GSK3B (NP_001139628.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2932
Swiss Prot	P49841
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	glycogen synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B_HUMAN.
Background	The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IP	1-2ug
IF