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GNAI3 Polyclonal Antibody

Lane 1: LO2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: HL60 cell lysates; Lane 4: HT-1080 cell lysates probed with GNAI3 Polyclonal Antibody, Unconjugated (bs-55087R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: LO2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: HL60 cell lysates; Lane 4: HT-1080 cell lysates probed with GNAI3 Polyclonal Antibody, Unconjugated (bs-55087R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: GNAI3 knockout (KO) HeLa cell lysates probed with GNAI3 Polyclonal Antibody, Unconjugated (bs-55087R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: GNAI3 knockout (KO) HeLa cell lysates probed with GNAI3 Polyclonal Antibody, Unconjugated (bs-55087R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • IP

Reactivity

  • Human
Overview
Catalog # bs-55087R
Product Name GNAI3 Polyclonal Antibody
Applications WB, IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 2773
Swiss Prot P08754
Subcellular location Cytoplasm, Cell membrane
Synonyms GNAI3;87U6;ARCND1
Background Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.
Application Dilution
WB 1:300-5000
IP 1-2ug

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