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CAPN2 Polyclonal Antibody

Lane 1: H460 cell lysates; Lane 2: A549 cell lysates; Lane 3: Hela cell lysates; Lane 4: Mouse kidney lysates; Lane 5: Mouse Lung lysates probed with CAPN2 Polyclonal Antibody, Unconjugated (bs-55030R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: H460 cell lysates; Lane 2: A549 cell lysates; Lane 3: Hela cell lysates; Lane 4: Mouse kidney lysates; Lane 5: Mouse Lung lysates probed with CAPN2 Polyclonal Antibody, Unconjugated (bs-55030R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: CAPN2 knockout (KO) HeLa cell lysates probed with CAPN2 Polyclonal Antibody, Unconjugated (bs-55030R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: CAPN2 knockout (KO) HeLa cell lysates probed with CAPN2 Polyclonal Antibody, Unconjugated (bs-55030R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CAPN2) polyclonal Antibody, Unconjugated (bs-55030R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CAPN2) polyclonal Antibody, Unconjugated (bs-55030R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

  • WB
  • IHC-P
  • IF(ICC)
  • IF

Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-55030R
Product Name CAPN2 Polyclonal Antibody
Applications WB, IHC-P, IF(ICC), IF
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human CAPN2 (NP_001739.2).
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 824
Swiss Prot P17655
Subcellular location Cytoplasm, Cell membrane
Synonyms CAPN2;CANP2;CANPL2;CANPml;mCANP;calpain 2
Background The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IF

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