MYL6(Tyr29+Ser30) Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse embryonic muscle labeled with Anti-phospho-MYL6(Tyr29+Ser30)Polyclonal Antibody, Unconjugated (bs-5478R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Sheep
Pig
Chicken

Overview
Catalog #	bs-5478R
Product Name	MYL6(Tyr29+Ser30) Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Sheep, Pig, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human MYL6 around the phosphorylation site of Tyr29+Ser30
Modification Site	Tyr29+Ser30
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4637
Subcellular location	Cytoplasm, Nucleus
Synonyms	ESMLC; LC 17; LC17; LC17 GI; LC17 NM; LC17A; LC17B; MLC 3; MLC-3; MLC1SM; MLC3; MLC3NM; MLC3SM; MYL 6; MYL6; MYL6_HUMAN; Myosin light chain 3; Myosin light chain 6 alkali smooth muscle and non muscle; Myosin light chain A3; Myosin light chain alkali 3; Myosin light polypeptide 6 alkali smooth muscle and non muscle; Myosin light polypeptide 6; Smooth muscle and non muscle myosin alkali light chain; Smooth muscle and nonmuscle myosin light chain alkali 6; 17 kDa myosin light chain.
Background	Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200