MCAM(Tyr641) Polyclonal Antibody

Lane 1:Siha cell lysates probed with MCAM(Tyr641) Polyclonal Antibody, Unconjugated (bs-5472R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human U2OS cell lysates; Lane 6: Human U87MG cell lysates probed with phospho-MCAM (Tyr641) Polyclonal Antibody, Unconjugated (bs-5472R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Human Huvec cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human U2OS cell lysates; Lane 6: Human U87MG cell lysates probed with phospho-MCAM (Tyr641) Polyclonal Antibody, Unconjugated (bs-5472R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human
Mouse

Overview
Catalog #	bs-5472R
Product Name	MCAM(Tyr641) Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human MCAM around the phosphorylation site of Tyr641
Modification Site	Tyr641
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4162
Subcellular location	Cell membrane
Synonyms	Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; MCAM; MUC18
Background	Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000