VLDL Receptor Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-VLDL Polyclonal Antibody, Unconjugated (bs-4228R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

HEK 239T cells probed with VLDL Receptor Polyclonal Antibody, Unconjugated (bs-4228R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Lane 1: Human A549 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human Siha cell lysates probed with VLDL Receptor Polyclonal Antibody, Unconjugated (bs-4228R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (VLDL Receptor) polyclonal Antibody, Unconjugated (bs-4228R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (VLDL Receptor) polyclonal Antibody, Unconjugated (bs-4228R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
FCM

Reactivity

Human
Mouse

Overview
Catalog #	bs-4228R
Product Name	VLDL Receptor Polyclonal Antibody
Applications	WB, ELISA, FCM
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human VLDL Receptor
Immunogen Range	551-650/873
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7436
Swiss Prot	P98155
Subcellular location	Cell membrane
Synonyms	CARMQ1; CHRMQ1; VLDLRCH; Very low-density lipoprotein receptor; VLDL receptor; VLDL-R; VLDLR
Background	Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100