GPR15 Polyclonal Antibody

$L1 human colon carcinoma lysates L2 rat brain lysates probed with Anti GPR15 Polyclonal Antibody, Unconjugated (bs-3699R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 40kD. Observed band size:40kD.\\n$

L1 human colon carcinoma lysates L2 rat brain lysates probed with Anti GPR15 Polyclonal Antibody, Unconjugated (bs-3699R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 40kD. Observed band size:40kD.\\n

Formalin-fixed and paraffin embedded rat stomach labeled with Rabbit Anti-GPR15 Polyclonal Antibody, Unconjugated(bs-3699R) 1:200 followed by conjugation to the secondary antibody and DAB staining

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Horse
Rabbit

Overview
Catalog #	bs-3699R
Product Name	GPR15 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GPR15
Immunogen Range	51-150/360
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2838
Swiss Prot	P49685
Subcellular location	Cytoplasm, Cell membrane
Synonyms	BOB; G-protein coupled receptor 15; Brother of Bonzo; GPR15
Background	GPR15 is a probable chemokine receptor,its expression has been reported in CD4(+) T lymphocytes, alveolar macrophages, and the basal surface of the small intestinal epithelium. ESTs have been isolated from normal brain and kidney cancer libraries.This gene encodes a G protein-coupled receptor that acts as a chemokine receptor for human immunodeficiency virus type 1 and 2. The encoded protein localizes to the cell membrane. [provided by RefSeq, Nov 2012].
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500