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eNOS (Thr113) Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Rabbit
Overview
Catalog # bs-3589R-Cy5
Product Name eNOS (Thr113) Polyclonal Antibody, Cy5 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity This phosphorylation site is homologous across the listed species; this phosphorylation site does not exist in human due to a Thr-Ser substitution.
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser113
Modification Site Thr113
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 18127
Swiss Prot P70313
Subcellular location Cytoplasm, Cell membrane
Synonyms eNOS; Nos-3; ecNOS; 2310065A03Rik; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; Nos3
Background Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200