TBK1 (Ser172) Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-TBK1 (Ser172) Polyclonal Antibody, Unconjugated(bs-3440R) 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Human Raji cell lysates; Lane 2: Human HeLa cell lysates probed with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (bs-3440R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Raji cell lysates; Lane 2: Human HeLa cell lysates probed with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (bs-3440R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bs-3440R
Product Name	TBK1 (Ser172) Polyclonal Antibody
Applications	WB
Specificity	This phosphorylation site is homologous across the listed species.
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human TBK1 around the phosphorylation site of Ser172
Modification Site	Ser172
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	29110
Swiss Prot	Q9UHD2
Subcellular location	Cytoplasm
Synonyms	NAK; T2K; Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; TANK-binding kinase 1; TBK1
Background	Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. Phosphorylates Borna disease virus (BDV) P protein.
Application Dilution
WB	1:500-2000