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FAK(Tyr576 + Tyr577) Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-3162R-Cy3
Product Name FAK(Tyr576 + Tyr577) Polyclonal Antibody, Cy3 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity These phosphorylation sites are homologous to Tyr614 + Tyr615 in Mouse.
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human FAK around the phosphorylation site of Tyr576/577
Modification Site Tyr576 + Tyr577
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5747
Swiss Prot Q05397
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; PTK2
Background Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200