Ephrin B (Ser300) Polyclonal Antibody

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with phospho-Ephrin B (Ser300) Polyclonal Antibody, Unconjugated (bs-3128R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with phospho-Ephrin B (Ser300) Polyclonal Antibody, Unconjugated (bs-3128R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat
Bovine

Overview
Catalog #	bs-3128R
Product Name	Ephrin B (Ser300) Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat, Bovine
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Ser300
Modification Site	Ser300
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1947
Synonyms	Ephrin B phospho Y330; p-Ephrin B phospho Y330; CEK5 ligand; EFNB1; ELK L; EphrinB; Ephrin-B; ELK ligand; ELKL; EPH related receptor tyrosine kinase ligand 2; Ephrin B Precursor; Ephrin B1; Ephrin B2; LERK 2; LERK2; EFNB2_HUMAN.
Background	Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200