New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

ATF2 (Thr69/71) Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-3033R-Biotin
Product Name ATF2 (Thr69/71) Polyclonal Antibody, Biotin Conjugated
Applications ELISA, IHC-P, IHC-F
Specificity This antibody is specific for ATF2 in when phosphorylated at both the Thr69 and Thr71 residues
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Thr69/71
Modification Site Thr69/71
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 1386
Swiss Prot P15336
Subcellular location Nucleus
Synonyms ATF2 phospho T69 + T71; ATF2 phospho Thr69 + Thr71; p-ATF2 T69 + T71; p-ATF2 Thr69 + Thr71; CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN.
Background ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500