CRHR2 Polyclonal Antibody

Lane 1: Mouse Brain lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Mouse Kidney lysates; Lane 4: Mouse Heart cell lysates ; Lane 5: Mouse Muscle lysates probed with CRHR2 Polyclonal Antibody, Unconjugated (bs-2792R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human Hela cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL60 cell lysates probed with CRHR2 Polyclonal Antibody, Unconjugated (bs-2792R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Hela cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL60 cell lysates probed with CRHR2 Polyclonal Antibody, Unconjugated (bs-2792R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-2792R
Product Name	CRHR2 Polyclonal Antibody
Applications	WB, ELISA, IF(IHC-F), IF(ICC)
Specificity	This antibody may cross react with CRHR1.
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CRHR2
Immunogen Range	51-150/411
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1395
Swiss Prot	Q13324
Subcellular location	Cell membrane
Synonyms	CRF2; CRFR2; CRF-RB; HM-CRF; Corticotropin-releasing factor receptor 2; CRF-R-2; CRF-R2; CRFR-2; Corticotropin-releasing hormone receptor 2; CRH-R-2; CRH-R2; CRHR2; CRF2R; CRH2R
Background	G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200