CLEC5A Polyclonal Antibody

$Antigen: bs-2663P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-2663P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

$Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-CLEC5A\/MDL1 Polyclonal Antibody (bs-2663R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-CLEC5A\/MDL1 Polyclonal Antibody (bs-2663R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

U937 cells probed with Rabbit Anti-CLEC5A Polyclonal Antibody, FITC Conjugated (bs-2663R-FITC) at 1:50 for 40 minutes (green)compared to control cells (blue).

Mouse spleen lysates probed with Rabbit Anti-CLEC5A Polyclonal Antibody, Unconjugated (bs-2663R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CLEC5A Polyclonal Antibody, Unconjugated (bs-2663R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CLEC5A Polyclonal Antibody, Unconjugated (bs-2663R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Rat Spinal cord tissue lysates probed with CLEC5A Polyclonal Antibody, Unconjugated (bs-2663R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Rat Spinal cord tissue lysates probed with CLEC5A Polyclonal Antibody, Unconjugated (bs-2663R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-2663R
Product Name	CLEC5A Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse CLEC5A
Immunogen Range	101-190/190
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23845
Swiss Prot	Q9R007
Subcellular location	Cell membrane
Synonyms	Mdl1; Ly100; MDL-1; Clecsf5; C-type lectin domain family 5 member A; C-type lectin superfamily member 5; Myeloid DAP12-associating lectin 1; Clec5a
Background	Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation. Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through phosphylation of TYROBP, this interaction does not result in viral entry but stimulates proinflammatory cytokine release.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500