MD-2 Polyclonal Antibody

$Lane 1: Human U87MG cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U251 cell lysates probed with MD2 Polyclonal Antibody, Unconjugated (bs-2662R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human U87MG cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human U251 cell lysates probed with MD2 Polyclonal Antibody, Unconjugated (bs-2662R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Cow
Horse
Rabbit

Overview
Catalog #	bs-2662R
Product Name	MD-2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Cow, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human LY-96
Immunogen Range	81-160/160
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23643
Subcellular location	Secreted, Extracellular matrix
Synonyms	Lymphocyte antigen 96; Ly-96; ESOP-1; Protein MD-2; LY96; ESOP1; MD2
Background	Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200