IL10RB Polyclonal Antibody

Lane 1: mouse kidney lysates Lane 2: mouse lung lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-2602R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 39kD. Observed band size: 39kD.

Lane 1: HepG2 cell lysates; Lane 2: Mouse Lung lysates; Lane 3: Rat Lung lysates probed with IL10RB Polyclonal Antibody, Unconjugated (bs-2602R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-2602R
Product Name	IL10RB Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IL10RB
Immunogen Range	21-120/325
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3588
Swiss Prot	Q08334
Subcellular location	Cell membrane
Synonyms	Interleukin-10 receptor subunit beta; IL-10 receptor subunit beta; IL-10R subunit beta; IL-10RB; Cytokine receptor class-II member 4; Cytokine receptor family 2 member 4; CRF2-4; Interleukin-10 receptor subunit 2; IL-10R subunit 2; IL-10R2; CDw210b; IL10RB; CRFB4; D21S58; D21S66
Background	Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200