DDB2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat intestine labeled with Anti-DDB2 Polyclonal Antibody, Unconjugated (bs-2599R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.

Mouse Liver lysates probed with DDB2 Polyclonal Antibody, Unconjugated (bs-2599R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$SP2\/0 lysates probed with DDB2 Polyclonal Antibody, Unconjugated (bs-2599R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.$

SP2\/0 lysates probed with DDB2 Polyclonal Antibody, Unconjugated (bs-2599R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Cow
Horse
Rabbit

Overview
Catalog #	bs-2599R
Product Name	DDB2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody may have a minor secondary cross-reactivity to TNFAIP3-interacting protein 2 due to a 63% non-sequential sequence similarity in the immunogen region.
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Cow, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DDB2
Immunogen Range	201-300/427
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1643
Swiss Prot	Q92466
Subcellular location	Nucleus
Synonyms	DDBB; UV-DDB2; DNA damage-binding protein 2; DDB p48 subunit; Damage-specific DNA-binding protein 2; UV-damaged DNA-binding protein 2; UV-DDB 2; DDB2
Background	Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200