CD32 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human glioma labeled with Rabbit Anti-CD32 Polyclonal Antibody, Unconjugated (bs-2573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded human glioma labeled with Rabbit Anti-CD32 Polyclonal Antibody, Unconjugated (bs-2573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

K562 lysates probed with Anti-CD32 Polyclonal Antibody, Unconjugated (bs-2573R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

Human U937 cells probed with CD32 Polyclonal Antibody, Unconjugated (bs-2573R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P

Reactivity

Human

Overview
Catalog #	bs-2573R
Product Name	CD32 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD32
Immunogen Range	201-300/317
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2212
Swiss Prot	P12318
Subcellular location	Cell membrane
Synonyms	CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1; Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; FCGR2A
Background	Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400