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EphB6 Polyclonal Antibody

Mouse kidney cells probed with Rabbit Anti-EphB6 Polyclonal Antibody (bs-2571R) at 1:50 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).
Mouse kidney cells probed with Rabbit Anti-EphB6 Polyclonal Antibody (bs-2571R) at 1:50 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).
Lane 1: U2os cell lysates probed with EphB6 Polyclonal Antibody, Unconjugated (bs-2571R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: U2os cell lysates probed with EphB6 Polyclonal Antibody, Unconjugated (bs-2571R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA

Reactivity

  • Human
Overview
Catalog # bs-2571R
Product Name EphB6 Polyclonal Antibody
Applications WB, ELISA
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EphB6
Immunogen Range 551-650/1021
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2051
Swiss Prot O15197
Subcellular location Secreted, Cell membrane
Synonyms HEP; Ephrin type-B receptor 6; Tyrosine-protein kinase-defective receptor EPH-6; EPHB6
Background Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000

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