FTO Polyclonal Antibody

$Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Rat Adrenal gland tissue lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human MOLT4 cell lysates probed with FTO Polyclonal Antibody, Unconjugated (bs-25583R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Adrenal gland tissue lysates; Lane 2: Rat Adrenal gland tissue lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human MOLT4 cell lysates probed with FTO Polyclonal Antibody, Unconjugated (bs-25583R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-25583R
Product Name	FTO Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FTO
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79068
Swiss Prot	Q9C0B1
Subcellular location	Nucleus
Synonyms	Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; FTO_HUMAN; Protein fatso.
Application Dilution
WB	=1:500-2000
ELISA	ELISA=1:5000-10000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500