Bim Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Bim) Polyclonal Antibody, Unconjugated (bs-25552R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Bim) Polyclonal Antibody, Unconjugated (bs-25552R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

ELISA
IHC-P

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig

Overview
Catalog #	bs-25552R
Product Name	Bim Polyclonal Antibody
Applications	ELISA, IHC-P
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Bim
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10018
Swiss Prot	O43521
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Bcl-2 interacting mediator of cell death; Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death; BCL2L11; BAM; BIM-alpha6; BIM-beta6; BIM-beta7; BimEL; BimL; BOD; B2L11_HUMAN.
Application Dilution
ELISA	=1:5000-10000
IHC-P	IHC-P=1:100-500