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CD30 Polyclonal Antibody, FITC Conjugated

Raji probed with CD30 Polyclonal Antibody, FITC Conjugated (bs-2495R-FITC) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).
Raji probed with CD30 Polyclonal Antibody, FITC Conjugated (bs-2495R-FITC) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).
K562 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD30 Polyclonal Antibody(bs-2495R-FITC)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
K562 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD30 Polyclonal Antibody(bs-2495R-FITC)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raji cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nCD30 Antibody\uff08bs-2495R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Raji cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nCD30 Antibody\uff08bs-2495R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Pig

Predicted Reactivity

  • Rabbit
Overview
Catalog # bs-2495R-FITC
Product Name CD30 Polyclonal Antibody, FITC Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Pig
Predicted Reactivity Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD30
Immunogen Range 131-230/595
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 943
Swiss Prot P28908
Subcellular location Cytoplasm, Cell membrane
Synonyms CD3; Ki-1; D1S166E; Tumor necrosis factor receptor superfamily member 8; CD3L receptor; Ki-1 antigen; Lymphocyte activation antigen CD3; TNFRSF8
Background Receptor for TNFSF8/CD3L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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