NCR2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-NCR2 Polyclonal Antibody, Unconjugated (bs-2420R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\n$

Paraformaldehyde-fixed, paraffin embedded human spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-NCR2 Polyclonal Antibody, Unconjugated (bs-2420R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\n

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bs-2420R
Product Name	NCR2 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD336/NCR2
Immunogen Range	201-276/276
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9436
Swiss Prot	O95944
Subcellular location	Cell membrane
Synonyms	LY95; CD336; NKP44; NK-p44; dJ149M18.1; Natural cytotoxicity triggering receptor 2; Lymphocyte antigen 95 homolog; NK cell-activating receptor; Natural killer cell p44-related protein; NCR2
Background	Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200