alpha Internexin Polyclonal Antibody

$Antigen: bs-2408P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-2408P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti NF66\/alpha Internexin Polyclonal Antibody, Unconjugated (bs-2408R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti NF66\/alpha Internexin Polyclonal Antibody, Unconjugated (bs-2408R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human HeLa cell lysates probed with alpha Internexin Polyclonal Antibody, Unconjugated (bs-2408R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human HeLa cell lysates probed with alpha Internexin Polyclonal Antibody, Unconjugated (bs-2408R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Pig
Horse

Overview
Catalog #	bs-2408R
Product Name	alpha Internexin Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human alpha Internexin
Immunogen Range	251-350/499
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9118
Swiss Prot	Q16352
Subcellular location	Cytoplasm
Synonyms	NEF5; NF-66; TXBP-1; Alpha-internexin; Alpha-Inx; 66 kDa neurofilament protein; Neurofilament-66; Neurofilament 5; INA
Background	Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200