BRD2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (BRD2) Polyclonal Antibody, Unconjugated (bs-21544R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (BRD2) Polyclonal Antibody, Unconjugated (bs-21544R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human K562 cell lysates probed with BRD2 Polyclonal Antibody, Unconjugated (bs-21544R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human K562 cell lysates probed with BRD2 Polyclonal Antibody, Unconjugated (bs-21544R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF
ICC

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Horse

Overview
Catalog #	bs-21544R
Product Name	BRD2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF, ICC
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human BRD2
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6046
Swiss Prot	P25440
Subcellular location	Nucleus
Synonyms	Bromodomain-containing protein 2; KIAA9001; RING3; O27.1.1; Really interesting new gene 3 protein; BRD2_HUMAN;
Application Dilution
WB	=1:500-2000
ELISA	ELISA=1:5000-10000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500
ICC	ICC=1:100-500