phospho-Munc18-1 (Thr574) Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human cerebellum ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-Munc18-1 (Thr574)) Polyclonal Antibody, Unconjugated (bs-19105R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human cerebellum ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (phospho-Munc18-1 (Thr574)) Polyclonal Antibody, Unconjugated (bs-19105R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF
ICC

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-19105R
Product Name	phospho-Munc18-1 (Thr574) Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF, ICC
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthesised phosphopeptide derived from human Munc18-1 around the phosphorylation site of Thr574
Modification Site	Thr574
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6812
Swiss Prot	P61764
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Munc18-1 (phospho T574); p-Munc18-1 (phospho T574); FLJ37475; Munc 18 1; Munc 18a; MUNC18 1; N-Sec1; Neuronal SEC1; NSec1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; Rb sec1; RBSEC1; STXB1_HUMAN; STXBP1; Syntaxin binding protein 1; Syntaxin-binding protein 1; Unc 18 homolog; Unc 18A; Unc-18A; Unc18 1; UNC18; Unc18-1.
Application Dilution
ELISA	=1:5000-10000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500
ICC	ICC=1:100-500