DUSP1/MKP1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-DUSP1\/MKP-1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:200, followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-DUSP1\/MKP-1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DUSP1\/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DUSP1\/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Lane 1: Mouse Pancreas lysates probed with DUSP1\/MKP1 Polyclonal Antibody, Unconjugated (bs1851R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Pancreas lysates probed with DUSP1\/MKP1 Polyclonal Antibody, Unconjugated (bs1851R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Chicken
Rabbit

Overview
Catalog #	bs-1851R
Product Name	DUSP1/MKP1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody may interact with DUSP4/MKP2
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DUSP1
Immunogen Range	151-250/367
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1843
Swiss Prot	P28562
Subcellular location	Nucleus
Synonyms	HVH1; MKP1; CL100; MKP-1; PTPN10; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; Protein-tyrosine phosphatase CL100; DUSP1; VH1
Background	Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200