KLF10 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human brain glioblastoma labeled with Anti TIEG1\/KLF10 Polyclonal Antibody, Unconjugated (bs-1838R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human brain glioblastoma labeled with Anti TIEG1\/KLF10 Polyclonal Antibody, Unconjugated (bs-1838R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin-embedded Mouse Testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TIEG1\/KLF10 Polyclonal Antibody, Unconjugated (bs-1838R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin-embedded Mouse Testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TIEG1\/KLF10 Polyclonal Antibody, Unconjugated (bs-1838R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (KLF10) polyclonal Antibody, Unconjugated (bs-1838R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (KLF10) polyclonal Antibody, Unconjugated (bs-1838R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

ELISA
ICC

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bs-1838R
Product Name	KLF10 Polyclonal Antibody
Applications	ELISA, ICC
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TIEG1
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7071
Swiss Prot	Q13118
Subcellular location	Nucleus
Synonyms	TIEG1; TGF-beta inducible early growth response gene 1; Transforming growth factor-beta-inducible early growth response protein 1; Krueppel-like factor 10; TIEG-1; EGR-alpha; KLF10; TIEG; GDNF-inducible factor; Transcription factor GIF; mGIF; KLF10_HUMAN.
Application Dilution
ELISA	=1:5000-10000
ICC	ICC=1:25