Transferrin receptor Polyclonal Antibody

$HL-60 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Transferrin receptor Antibody\uff08bs-1782R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).$

HL-60 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Transferrin receptor Antibody\uff08bs-1782R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Applications

ELISA
FCM

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig
Horse
Chicken

Overview
Catalog #	bs-1782R
Product Name	Transferrin receptor Polyclonal Antibody
Applications	ELISA, FCM
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Transferrin receptor
Immunogen Range	21-120/760
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7037
Swiss Prot	P02786
Subcellular location	Cell membrane
Synonyms	Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; p90; TFRC
Background	Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100