Follistatin Polyclonal Antibody

$Antigen: bs-1773P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-1773P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti-Follistatin Polyclonal Antibody (bs-1773R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Lane 1: HCCLM3 lysates; probed with Follistatin Polyclonal Antibody, Unconjugated (bs-1773R) at 1:300 dilution and 4˚Covernight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Pituitary tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Rat Pituitary tissue lysates; Lane 4: Rat Placenta tissue lysates ; Lane 5: Rat Breast tissue lysates probed with Follistatin Polyclonal Antibody, Unconjugated (bs-1773R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Pituitary tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Rat Pituitary tissue lysates; Lane 4: Rat Placenta tissue lysates ; Lane 5: Rat Breast tissue lysates probed with Follistatin Polyclonal Antibody, Unconjugated (bs-1773R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-1773R
Product Name	Follistatin Polyclonal Antibody
Applications	ELISA, IHC-P
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Follistatin
Immunogen Range	151-250/344
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10468
Swiss Prot	P19883
Subcellular location	Secreted
Synonyms	FS; Follistatin; Activin-binding protein; FST
Background	Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400