NCX1/SLC8A1 Polyclonal Antibody

$Lane 1: mouse kidney lysates Lane 2: mouse liver lysates probed with Anti NCX1\/SLC8A1 Polyclonal Antibody, Unconjugated (bs-1550R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 106kD. Observed band size: 106kD.$

Lane 1: mouse kidney lysates Lane 2: mouse liver lysates probed with Anti NCX1\/SLC8A1 Polyclonal Antibody, Unconjugated (bs-1550R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 106kD. Observed band size: 106kD.

$Lane 1: Human U251 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates probed with NCX1\/SLC8A1 Polyclonal Antibody, Unconjugated (bs-1550R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human U251 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human A549 cell lysates probed with NCX1\/SLC8A1 Polyclonal Antibody, Unconjugated (bs-1550R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
IP

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Chicken
Guinea Pig

Overview
Catalog #	bs-1550R
Product Name	NCX1/SLC8A1 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Chicken, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NCX1
Immunogen Range	801-900/971
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6546
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Na+/Ca2+exchanger 1; CNC; DKFZp779F0871; MGC119581 ; FLJ37694; FLJ43417; Na+/Ca2+ exchange protein 1; Na+/Ca2+ exchanger; NCX 1; NCX; SLC8A1; SLC8A1 protein ; Sodium Calcium Exchanger; Sodium/calcium exchanger 1; Solute carrier family 8 member 1.
Background	In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma(plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM].
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
IP	1-2ug