GPCR EX33 Polyclonal Antibody

Mouse thymus lysates probed with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

$Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

$Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded human glioma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded human glioma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

$Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Mouse Spinal Cord lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Mouse Spinal Cord lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

U937 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GPR84 Polyclonal Antibody(bs-15353R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rabbit

Predicted Reactivity

Overview
Catalog #	bs-15353R
Product Name	GPCR EX33 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rabbit
Predicted Reactivity	Cow, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GPCR EX33 protein
Immunogen Range	21-120/369
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	53831
Swiss Prot	Q9NQS5
Subcellular location	Cell membrane
Synonyms	EX33; GPCR4; G-protein coupled receptor 84; Inflammation-related G-protein coupled receptor EX33; GPR84
Background	Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500