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CXCL10 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat lung labeled with Anti-CXCL10 Polyclonal Antibody, Unconjugated (bs-1502R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat lung labeled with Anti-CXCL10 Polyclonal Antibody, Unconjugated (bs-1502R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Raw264.7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL10 Polyclonal Antibody(bs-1502R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raw264.7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL10 Polyclonal Antibody(bs-1502R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-1502R
Product Name CXCL10 Polyclonal Antibody
Applications ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse CXCL10
Immunogen Range 35-98/98
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 15945
Swiss Prot P17515
Subcellular location Secreted
Synonyms C7; IP10; CRG-2; INP1; IP-10; Ifi1; mob-1; Scyb1; gIP-1; C-X-C motif chemokine 1; 1 kDa interferon gamma-induced protein; Gamma-IP1; Interferon-gamma induced protein CRG-2; Small-inducible cytokine B1; Cxcl1; Crg2
Background In addition to its role as a proinflammatory cytokine, may participate in T-cell effector function and perhaps T-cell development.
Application Dilution
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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