ELK1 Polyclonal Antibody

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-ELK1 Polyclonal Antibody, Unconjugated (bs-1398R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Mouse brain lysates probed with ELK1 Polyclonal Antibody, Unconjugated (bs-1398R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Blank control:Hela. \r\nPrimary Antibody (green line): Rabbit Anti-ELK1 antibody (bs-1398R) \r\nDilution: 1ug\/Test; \r\nProtocol\r\nThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.$

Blank control:Hela. \r\nPrimary Antibody (green line): Rabbit Anti-ELK1 antibody (bs-1398R) \r\nDilution: 1ug\/Test; \r\nProtocol\r\nThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Horse

Overview
Catalog #	bs-1398R
Product Name	ELK1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ELK1
Immunogen Range	301-428/428
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2002
Swiss Prot	P19419
Subcellular location	Nucleus
Synonyms	ETS domain-containing protein Elk-1; ELK1
Background	Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200