Apolipoprotein J Polyclonal Antibody

$Formalin-fixed and paraffin embedded tumor tissue labeled with Anti-APOJ\/Clusterin-\u03b1\u03b2 Polyclonal Antibody, Unconjugated (bs-1354R) followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded tumor tissue labeled with Anti-APOJ\/Clusterin-\u03b1\u03b2 Polyclonal Antibody, Unconjugated (bs-1354R) followed by conjugation to the secondary antibody and DAB staining

$Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti APOJ\/CLUSTERIN Polyclonal Antibody, Unconjugated (bs-1354R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 50kD. Observed band size: 50kD$

Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti APOJ\/CLUSTERIN Polyclonal Antibody, Unconjugated (bs-1354R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 50kD. Observed band size: 50kD

$Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-APOJ\/Clusterin Polyclonal Antibody, Unconjugated (bs-1354R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-APOJ\/Clusterin Polyclonal Antibody, Unconjugated (bs-1354R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded rat testis labeled with Anti-APOJ\/Clusterin Polyclonal Antibody, Unconjugated (bs-1354R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat testis labeled with Anti-APOJ\/Clusterin Polyclonal Antibody, Unconjugated (bs-1354R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse liver lysates probed with Rabbit Anti-Apolipoprotein J Polyclonal Antibody, Unconjugated (bs-1354R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Stomach lysates probed with Apolipoprotein J Polyclonal Antibody, Unconjugated (bs-1354R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Brain Lysates; Lane 2: Mouse Testis Lysates. Probed with APOJ polyclonal Antibody, unconjugated (bs-1531R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Brain Lysates; Lane 2: Mouse Testis Lysates. Probed with APOJ polyclonal Antibody, unconjugated (bs-1531R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Others

Overview
Catalog #	bs-1354R
Product Name	Apolipoprotein J Polyclonal Antibody
Applications	ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Others
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Apolipoprotein J
Immunogen Range	331-449/449
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1191
Swiss Prot	P10909
Subcellular location	Secreted
Synonyms	CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-4; TRPM2; TRPM-2; NA1/NA2; Clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Complement cytolysis inhibitor; Complement-associated protein SP-4,4; Ku7-binding protein 1; Testosterone-repressed prostate message 2; CLU
Background	Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC7. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200