GLYR1/NP60 Polyclonal Antibody

$Lane 1: Liver lysates probed with GLYR1\/NP60 Polyclonal Antibody, Unconjugated (bs-13451R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Liver lysates probed with GLYR1\/NP60 Polyclonal Antibody, Unconjugated (bs-13451R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Uterus lysates probed with GLYR1\/NP60 Polyclonal Antibody, Unconjugated (bs-13451R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Uterus lysates probed with GLYR1\/NP60 Polyclonal Antibody, Unconjugated (bs-13451R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Hela cell lysates; Lane 2: Mouse Stomach lysates; Lane 3: Mouse Liver lysates; Lane 4: Mouse Kidney lysates probed with GLYR1\/NP60 Polyclonal Antibody, Unconjugated (bs-13451R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Hela cell lysates; Lane 2: Mouse Stomach lysates; Lane 3: Mouse Liver lysates; Lane 4: Mouse Kidney lysates probed with GLYR1\/NP60 Polyclonal Antibody, Unconjugated (bs-13451R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Horse
Chicken
Monkey

Overview
Catalog #	bs-13451R
Product Name	GLYR1/NP60 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Horse, Chicken, Monkey
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NP60
Immunogen Range	21-120/553
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84656
Subcellular location	Nucleus
Synonyms	3 hydroxyisobutyrate dehydrogenase like protein; 3-hydroxyisobutyrate dehydrogenase-like protein; BM045; Cytokine like nuclear factor n pac; Cytokine-like nuclear factor N-PAC; Glyoxylate reductase 1 homolog; GLYR1; GLYR1_HUMAN; HIBDL; N-PAC; NP60; Nuclear protein 60 kDa; Nuclear protein NP60; Nuclear protein of 60 kDa; Putative oxidoreductase GLYR1.
Background	NP60 is a 553 amino acid nuclear protein that regulates the phosphorylation and activation of p38 alpha in response to stress. There are five isoforms of NP60 that are produced as a result of alternative splicing events.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500