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TPM2 Polyclonal Antibody

Formalin-fixed and paraffin-embedded mouse stomach labeled with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:200 followed by incubation with secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded mouse stomach labeled with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:200 followed by incubation with secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human breast carcinoma labeled with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:200 followed by incubation with secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human breast carcinoma labeled with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:200 followed by incubation with secondary antibody and DAB staining
Lane 1: Muscle lysates probed with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Muscle lysates probed with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Small intestine lysates probed with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Small intestine lysates probed with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Chicken
  • Rabbit
Overview
Catalog # bs-1243R
Product Name TPM2 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TPM2
Immunogen Range 11-130/284
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7169
Swiss Prot P07951
Subcellular location Cytoplasm
Synonyms Tropomyosin beta chain; Beta-tropomyosin; Tropomyosin-2; TPM2; TMSB; DA1A; DA2B; NEM4; AMCD1; HEL-S-273
Background Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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