P2Y10 Polyclonal Antibody

$HepG2 Cell lysates probed with P2Y10 Polyclonal Antibody, unconjugated (bs-12070R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

HepG2 Cell lysates probed with P2Y10 Polyclonal Antibody, unconjugated (bs-12070R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
ELISA
FCM
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Horse
Rabbit

Overview
Catalog #	bs-12070R
Product Name	P2Y10 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human P2Y10
Immunogen Range	151-250/339
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	27334
Swiss Prot	O00398
Subcellular location	Cell membrane
Synonyms	G protein coupled purinergic receptor P2Y10; P2ry10; P2Y like receptor; P2Y purinoceptor 10; P2Y-like receptor; P2Y10; P2Y10_HUMAN; Purinergic receptor P2Y G protein coupled 10; Putative P2Y purinoceptor 10.
Background	Nucleotides are important extracellular signaling molecules that mediate several events, such as cell proliferation, differentiation, chemotaxis and cytokine release. The P2 receptor family is activated by the binding of nucleotides and is divided into two subfamilies, designated P2X and P2Y. The P2Y receptor family are G protein-coupled receptors that mediate the effects of extracellular nucleotides, primarily through the activation of phospholipase C (PLC). To some extent, the P2Y receptors can also activate potassium channels or, alternatively, inhibit adenylate cyclase and N-type calcium channels in response to extracellular nucleotides. P2Y10 (purinergic receptor P2Y, G-protein coupled, 10), also known as P2RY10, is a 339 amino acid multi-pass membrane protein that is thought to act as a receptor for purines coupled to G-proteins. P2Y10 is found at low levels in blood leukocytes and is upregulated during promyelocytic cell differentiation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400