Frizzled 9/CD349 Polyclonal Antibody

$Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human MCF-7 cell lysates probed with Frizzled 9 Polyclonal Antibody, Unconjugated (bs-11842R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human MCF-7 cell lysates probed with Frizzled 9 Polyclonal Antibody, Unconjugated (bs-11842R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IF(ICC)
ICC

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bs-11842R
Product Name	Frizzled 9/CD349 Polyclonal Antibody
Applications	WB, ELISA, IF(ICC), ICC
Specificity	This antibody may have slight cross reactivity with Frizzled 10.
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Frizzled 9/CD349
Immunogen Range	185-270/591
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8326
Swiss Prot	O00144
Subcellular location	Cell membrane
Synonyms	FZD3; CD349; Frizzled-9; Fz-9; hFz9; FzE6; FZD9
Background	Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Application Dilution
WB	1:500-2000
ELISA	1:500-1000
IF(ICC)	1:50-200
ICC	1:100-500