PNUTS/PPP1R10 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-PNUTS\/PPP1R10 Polyclonal Antibody, Unconjugated (bs-11666R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-PNUTS\/PPP1R10 Polyclonal Antibody, Unconjugated (bs-11666R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded human kidney labeled with Anti-PNUTS\/PPP1R10 Polyclonal Antibody Unconjugated (bs-11666R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human kidney labeled with Anti-PNUTS\/PPP1R10 Polyclonal Antibody Unconjugated (bs-11666R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Mouse Testis lysates probed with PPP1R10 Polyclonal Antibody, Unconjugated (bs-11666R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HepG2 cell lysates probed with PPP1R10 Polyclonal Antibody, Unconjugated (bs-11666R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HepG2 cell lysates probed with PPP1R10 Polyclonal Antibody, Unconjugated (bs-11666R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig

Overview
Catalog #	bs-11666R
Product Name	PNUTS/PPP1R10 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PNUTS/PPP1R10
Immunogen Range	151-250/940
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Nucleus
Synonyms	CAT 53; CAT53; FB 19; FB19; FB19 protein; MHC class I region proline rich protein CAT53; p99; Phosphatase 1 nuclear targeting subunit; Phosphatase nuclear targeting subunit; PNUTS; PP1 binding protein of 114 kDa; PP1R10; Protein phosphatase 1 regulatory inhibitor subunit 10; Protein phosphatase 1 regulatory subunit 10; Serine/threonine protein phosphatase 1 regulatory subunit 10; PP1RA_HUMAN.
Background	Eukaryotic protein phosphorylation and dephosphorylation on serine and threonine residues regulates numerous cell functions, including division, homeostasis and apoptosis. A group of proteins that play a major role in this process are the serine/threonine protein phosphatases. Protein phosphatase (PP) holoenzyme is a trimeric complex that contains a regulatory subunit, a variable subunit and a catalytic subunit. Families of PP catalytic subunits include PP1, PP2A, PP2B, PP2C, PPX and PP5. Regulatory subunits include nuclear inhibitor of PP1 (NIPP1), PP1 nuclear targeting subunit (PNUTS), PP2A-A, PP2A-B, PP2A-B56, PP2A-C, PP2B-B and PR48. PNUTS, also designated CAT53 or FB19, is encoded by the gene PPP1R10. PNUTS acts as an inhibitor for the phosphatase activity of PP1 Alpha and PP1 Gamma. It is a nuclear protein primarily detected in nucleoplasmic bodies and within nucleoli. PNUTS expression levels are highest in brain, heart, lung, placenta, liver, kidney, pancreas and skeletal muscle.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500