A2LD1/GGACT Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-A2LD1\/GGACT Polyclonal Antibody, Unconjugated (bs-11381R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-A2LD1\/GGACT Polyclonal Antibody, Unconjugated (bs-11381R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.

$Mouse Liver lysates probed with A2LD1\/GGACT Polyclonal Antibody, Unconjugated (bs-11381R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Mouse Liver lysates probed with A2LD1\/GGACT Polyclonal Antibody, Unconjugated (bs-11381R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with A2LD1\/GGACT Polyclonal Antibody, Unconjugated (bs-11381R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with A2LD1\/GGACT Polyclonal Antibody, Unconjugated (bs-11381R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Horse
Rabbit

Overview
Catalog #	bs-11381R
Product Name	A2LD1/GGACT Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human A2LD1
Immunogen Range	1-50/153
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	87769
Swiss Prot	Q9BVM4
Subcellular location	Secreted, Extracellular matrix
Synonyms	A2LD1; Gamma-glutamylaminecyclotransferase; GGACT; AIG2-like domain-containing protein 1; Gamma-glutamylamine cyclotransferase
Background	Contributes to degradation of proteins cross-linked by transglutaminases. Degrades the cross-link between a lysine and a glutamic acid residue from two proteins that have been cross-linked by transglutaminases. Catalyzes the formation of 5-oxoproline from L-gamma-glutamyl-L-epsilon-lysine. Inactive with L-gamma-glutamyl-alpha-amino acid substrates such as L-gamma-glutamyl-L-alpha-cysteine and L-gamma-glutamyl-L-alpha-alanine.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500