New to Bioss? Enjoy 35% of your first order. Use code "FirstOrder35" - Offer valid for new U.S. Customers on direct orders only

SRD5A1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2) Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P

Reactivity

  • Human
  • Bovine
  • Sheep
  • Others

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Horse
  • Chicken
Overview
Catalog # bs-11308R-HRP
Product Name SRD5A1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2) Polyclonal Antibody, HRP Conjugated
Applications WB, ELISA, IHC-P
Reactivity Human, Bovine, Sheep, Others
Predicted Reactivity Mouse, Rat, Dog, Horse, Chicken
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SRD5A1
Immunogen Range 101-200/259
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm, Cell membrane
Synonyms 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1; S5A1_HUMAN.
Background Steroid 5a-Reductase is an important enzyme in androgen physiology because it catalyzes the conversion of testosterone into the more potent 5a-dihydro-testosterone, which mediates androgen effects on target tissues. The enzyme exists as two isoforms: type 1, which is expressed mainly in the skin; and type 2, which is expressed mainly in the prostate. In cultured human skin cells, 5a-Reductase 1 shows heterogeneity of protein, and has different levels of transcriptional and translational expression. 5a-Reductase 1 is expressed in all portions of the hair follicle, whereas 5a-Reductase 2 is expressed only in mesenchymal portions. In addition, 5a-Reductase 1 is mainly expressed in human breast carcinoma and may play a role in the in situ production and actions of the potent androgen 5a-dihydrotestosterone, including inhibition of cancer cell proliferation in hormone-dependent human breast carcinoma. The 5a-Reductase-3a-hydroxysteroid dehydrogenase complex is present in the human brain, suggesting that the complex may be involved in the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400