GPR49/LGR5 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-GPR49\/LGR5 Polyclonal Antibody, Unconjugated (bs-1117R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-GPR49\/LGR5 Polyclonal Antibody, Unconjugated (bs-1117R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded mouse colon carcinoma labeled with Anti-GPR49\/LGR5 Polyclonal Antibody, Unconjugated (bs-1117R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded mouse colon carcinoma labeled with Anti-GPR49\/LGR5 Polyclonal Antibody, Unconjugated (bs-1117R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$MCF-7 cells probed with GPR49\/LGR5 Polyclonal Antibody, unconjugated (bs-1117R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)$

MCF-7 cells probed with GPR49\/LGR5 Polyclonal Antibody, unconjugated (bs-1117R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Chicken
Rabbit

Overview
Catalog #	bs-1117R
Product Name	GPR49/LGR5 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse GPR49
Immunogen Range	810-907/907
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	14160
Swiss Prot	Q9Z1P4
Subcellular location	Cell membrane
Synonyms	FEX; Gpr49; Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; Orphan G-protein coupled receptor FEX; Lgr5
Background	Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200