IL-28R Polyclonal Antibody

Formalin-fixed and paraffin embedded human larynx labeled with Rabbit Anti-IL-28R Polyclonal Antibody, Unconjugated (bs-11137R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Tissue\/cell: Human Laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min; Primary Incubation: IL-28R Polyclonal Antibody, Unconjugated (bs-11137R) at 1:200, overnight at 4\u00b0C. Followed by secondary antibody, DAB and counterstaining.$

Tissue\/cell: Human Laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min; Primary Incubation: IL-28R Polyclonal Antibody, Unconjugated (bs-11137R) at 1:200, overnight at 4\u00b0C. Followed by secondary antibody, DAB and counterstaining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig
Rabbit

Overview
Catalog #	bs-11137R
Product Name	IL-28R Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Specificity	Predicted to also work in Rat based on sequence similarity
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IL-28R
Immunogen Range	51-150/520
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	163702
Swiss Prot	Q8IU57
Subcellular location	Cell membrane
Synonyms	IFNLR; LICR2; IL28RA; CRF2/12; IL-28R1; Interferon lambda receptor 1; IFN-lambda receptor 1; IFN-lambda-R1; Cytokine receptor class-II member 12; Cytokine receptor family 2 member 12; CRF2-12; Interleukin-28 receptor subunit alpha; IL-28 receptor subunit alpha; IL-28R-alpha; IL-28RA; Likely interleukin or cytokine receptor 2; IFNLR1
Background	The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state. Determines the cell type specificity of the lambda interferon action. Shows a more restricted pattern of expression in the epithelial tissues thereby limiting responses to lambda interferons primarily to epithelial cells of the respiratory, gastrointestinal, and reproductive tracts. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500