GRK1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Lane 1: Human HUVEC cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Ramos cell lysates probed with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HUVEC cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Ramos cell lysates probed with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Chicken

Overview
Catalog #	bs-1082R
Product Name	GRK1 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GRK1
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6011
Swiss Prot	Q15835
Subcellular location	Cell membrane
Synonyms	G-protein coupled receptor kinase 1; GRK1; GPRK1; RK; Grk1; Rhok; RHODOPSIN KINASE; RK_HUMAN.
Application Dilution
WB	=1:500-2000
ELISA	ELISA=1:5000-10000