Desmin Polyclonal Antibody, FITC Conjugated

$Tissue\/cell: H9C2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37\u00b0C for 20 min; Incubation: Anti-Desmin Polyclonal Antibody, conjugated (bs-1026R-FITC) 1:50, 2 hours at 37\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Tissue\/cell: H9C2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum, C-0006) at 37\u00b0C for 20 min; Incubation: Anti-Desmin Polyclonal Antibody, conjugated (bs-1026R-FITC) 1:50, 2 hours at 37\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
FCM
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat
Chicken

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Rabbit
Guinea Pig

Overview
Catalog #	bs-1026R-FITC
Product Name	Desmin Polyclonal Antibody, FITC Conjugated
Applications	WB, FCM, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat, Chicken
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Rabbit, Guinea Pig
Specifications
Conjugation	FITC
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Desmin
Immunogen Range	261-360/470
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	1674
Swiss Prot	P17661
Subcellular location	Cytoplasm
Synonyms	CSM1; CSM2; LGMD2R; Desmin; DES
Background	Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200

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