CD93/C1qrp Polyclonal Antibody

$Mouse lung probed with Rabbit Anti-CD93\/C1qrp Polyclonal Antibody, Unconjugated (bs-10232R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.$

Mouse lung probed with Rabbit Anti-CD93\/C1qrp Polyclonal Antibody, Unconjugated (bs-10232R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.

$Tissue\/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;\r\nAntigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min;\r\nIncubation: CD93\/C1qrp Polyclonal Antibody, Unconjugated (bs-10232R) 1:200, overnight at 4\u00b0C, followed by conjugation to the secondary antibody and DAB staining.$

Tissue\/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;\r\nAntigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min;\r\nIncubation: CD93\/C1qrp Polyclonal Antibody, Unconjugated (bs-10232R) 1:200, overnight at 4\u00b0C, followed by conjugation to the secondary antibody and DAB staining.

$Tissue\/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded.\r\nAntigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min.\r\nIncubation: Anti-CD93 Polyclonal Antibody, Unconjugated (bs-10232R) 1:200, overnight at 4\u00b0C, followed by conjugation to the secondary antibody and DAB staining.$

Tissue\/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded.\r\nAntigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37\u2103 for 20 min.\r\nIncubation: Anti-CD93 Polyclonal Antibody, Unconjugated (bs-10232R) 1:200, overnight at 4\u00b0C, followed by conjugation to the secondary antibody and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog

Overview
Catalog #	bs-10232R
Product Name	CD93/C1qrp Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD93/C1qrp
Immunogen Range	56-160/652
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	22918
Swiss Prot	Q9NPY3
Subcellular location	Extracellular
Synonyms	C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1; Complement component C1q receptor; C1q/MBL/SPA receptor; C1qR; Complement component 1 q subcomponent receptor 1; Matrix-remodeling-associated protein 4; CD93
Background	Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500